冷诱导RNA结合蛋白参与小鼠对LPS的应答

doi:10.11843/j.issn.0366-6964.2014.08.021

Abstract

Abstract:

The objective of this paper was to explore if Cold-inducible RNA binding protein(CIRP) involved in the response of mice to LPS.Total 48 BALB/c mice were randomly divided into 4 groups，LPS was injected into abdominal cavity of each mouse in dose of 2.5，5，7.5 mg•kg^－1 body weight for 1-3 experimental groups.The 4th group(control group) was injected with the same volume saline solution in the same way with the above groups.Three mice were selected in each group and euthanized at 3，6，12 and 18 h post injection.The heart，liver，spleen and kidney of each mouse were collected.Real-time RT-PCR established in this study and immunohistochemistry were used to detect the transcription of CIRP mRNA and expression of CIRP protein in each organ.The results showed that the Cirp mRNA significantly increased in liver，spleen and kidney post LPS injection，while Cirp mRNA transcription in heart was inhibited at all time points.CIRP protein was highly expressed in all detected organs after LPS stimulation.In conclusion，the results in this paper demonstrated that CIRP involves in response to LPS in mice and present evidence to identify new feature of CIRP.

CLC Number:

S852.3

ZHOU Hong-miao，TANG Cheng，YUE Hua，TANG Jia-jia. Cold-inducible RNA Binding Protein Involves in Response to LPS in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1348-1354.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.08.021

https://www.xmsyxb.com/EN/Y2014/V45/I8/1348

References

［1］FORNACE A J Jr，ALAMO I Jr，HOLLANDER M C.DNA damage-inducible transcripts in mammalian cells［J］.Proc Natl Acad Sci U S A，1988，85(23):8800-8804.
［2］SHEIKH M S，CARRIER F，PAPATHANASIOU M A，et al.Identification of several human homologs of hamster DNA damage-inducible transcripts.Cloning and characterization of a novel UV-inducible cDNA that codes for a putative RNA-binding protein ［J］.J Biol Chem，1997，272(42)：26720-26726.
［3］NISHIYAMA H，ITOH K，KANEKO Y，et al.A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth ［J］.J Cell Biol，1997，137(4)：899-908.
［4］XUE J H，NONOGUCHI K，FUKUMOTO M，et al.Effects of ischemia and H₂O₂ on the cold stress protein CIRP expression in rat neuronal cells［J］.Free Radic Biol Med，1999，27(11-12):1238-1244.
［5］WELLMANN S，BÜHRER C，MODEREGGER E，et al.Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism［J］.J Cell Sci，2004，117(Pt 9):1785-1794.
［6］PAN F，ZARATE J，CHOUDHURY A，et al.Osmotic stress of salmon stimulates upregulation of a cold inducible RNA binding protein(CIRP) similar to that mammals and amphibians ［J］.Biochimine，2004，86(7)：451-461.
［7］SAKURAI T，ITOH K，HIGASHITSUJI H，et al.Cirp protects against tumor necrosis factor-α-induced apoptosis via activation of extracellular signal-regulated kinase［J］.Biochimica Biophy Acta，2006，1763(3)：290-295.
［8］SAITO K，FUKUDA N，MATSUMOTO T，et a1.Moderate low temperature preserves the stemness of neural stem cells and suppresses apoptosis of the cells via activation of the cold-inducible RNA binding protein［J］.Brain Res，2010，1358:20-29．
［9］RIETSCHEL E T，KIRIAE T，SCHADE F U，et al.Bacterial endotoxin：molecular relationships of structure to activity and function［J］.FASEB J ，1994，8(2)：217-225.
［10］BROCHU C，CABRITA M A，MELANSON B D，et al.NF-κB-dependent role for cold-inducible RNA binding protein in regulating interleukin 1β［J］.PLoS One，2013，8(2)：e57426.
［11］周鸿淼，岳华,汤承，等.脂多糖染毒小鼠稳定内参基因的筛选［J］.中国畜牧兽医，2013，40(10):68-72.
［12］LIVAK K J，SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-△△Ct method ［J］.Methods，2001，25(4)：402-408.
［13］蔡文琴，王波沄.实用免疫细胞化学与核酸分子杂交技术［M］.成都：四川科技出版社，1994.
［14］BUSTIN S A，NOLAN T.Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction［J］.J Biomol Tech，2004,15(3):155-166.
［15］BUSTIN S A，BENES V，NOLAN T，et al.Quantitative real-time RT-PCR-a perspective ［J］.J Mol Endocrinol，2005，34(3):597-601.
［16］KUBISTA M，ANDRADE J M，BENGTSSON M，et al.The real-time polymerase chain reaction［J］.Mol Aspects Med，2006，27(2-3):95-125.
［17］NEUVIANS T P，GASHAW I，SAUER C G，et al.Standardization strategy for quantitative PCR in human seminoma and normal testis［J］.J Biotechnol，2005，117(2)：163-171.
［18］GUO Y，XIAO P，LEI S，et al.How is mRNA expression predictive for protein expression? A correlation study on human circulating monocytes［J］.Acta Biochim Biophys Sin(Shanghai)，2008，40(5)：426-436.
［19］CHEN G，GHARIB T G，HUANG C C，et al.Discordant protein and mRNA expression in lung adenocarcinomas ［J］.Mol Cell Proteomics，2002，1(4):304-313.
［20］OSTLUNDA G，SONNHAMMER E L.Quality criteria for finding genes with high mRNA-protein expression correlation and coexpression correlation［J］.Gene,2012,497(2):228-236.
［21］GEDEON T，BOKES P.Delayed protein synthesis reduces the correlation between mRNA and protein fluctuations［J］.Biophys J，2012，103(3)：377-385.
［22］MASUDA T，ITOH K，HIGASHITSUJI H，et al.Cold-inducible RNA-binding protein(Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice［J］.Proc Natl Acad Sci U S A，2012，109(27)：10885-10890.
［23］ARTERO-CASTRO A，CALLEJAS F B，CASTELLVI J，et al.Cold-inducible RNA-binding protein bypasses replicative senescence in primary cells through extracellular signal-regulated kinase 1 and 2 activation ［J］.Mol Cell Biol，2009，29(7):1855-1868.
［24］SAKURAI T，ITOH K，HIGASHITSUJI H，et al.Cirp protects against tumor necrosis factor-α-induced apoptosis via activation of extracellular signal-regulated kinase［J］.Biochim Biophys Acta，2006，1763(3)：290-295.

[1]	XIAO Jinlong, WANG Hao, WAN Quan, SHEN Jue, ZHANG Bo, ZHAO Weiwei, DENG Jing, WANG Xi, ZHAO Ru, XIAO Peng, GAO Hong. Induction of IPEC-J2 Pyroptosis by E.coli HPI from Saba Pig via NLRP3/ASC/Caspase-1 Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5218-5227.
[2]	WANG Siying, ZOU Hong, SONG Zhenhui. The Role of Na⁺/H⁺ Exchanger Isoform 3 in Infectious Diarrhea and Its Activity Regulation Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3230-3241.
[3]	SHEN Tong, WANG Mengjie, WU Hua, LI Jinming, WANG Shuo, WU Xiaoqing, CHEN Xinlei, XING Qianwen, LIU Bo. Effect of Lycium ruthenicum Murray Anthocyanin on Hypoxia-induced Apoptosis in H9c2 Rat Cardiomyocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3490-3499.
[4]	ZHANG Haiyang, ZHU Qiuxiang, GAO Zhicheng, JIA Kun, WANG Jingyu, JI Jinzhao, LIANG Wuying, MO Ruiwen, LI Shoujun. Establishment of Acute Stress Model and Screening of Diagnostic Markers in Cats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1249-1260.
[5]	LIU Zelin, GUO Xiaoyan, LI Jiahuan, GAO Mingxing, CHENG Guofu, HU Xueying, ZHANG Wanpo, GU Changqin. Effect of the Nrf2 Pathway on Oxidative Stress Induced by Japanese Encephalitis Virus Infection of Mouse Neuroblastoma Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 317-327.
[6]	ZHANG Ruili, ZHANG Di, GUO Rong, CHEN Yang, LI Guangxing, HUANG Xiaodan. Selenium Deficiency Induced Thymocytes Apoptosis of Broilers via the TLR4/MyD88/NF-κB Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4035-4047.
[7]	CAO Mengyuan, CHEN Mingjie, WANG Chenyu, LI Yitao, YAN Xueqi, CHEN Jie, QI Yayin. Pathological Observation and Transcriptomic Difference Analysis from Mice Infected with Enterococcus faecalis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3160-3171.
[8]	BAI Yu, WANG Wu, WANG Bi, LI Ying, FENG Zili. The Cytotoxicity of Amyloid Fibrils Formed by Hen Egg-White Lysozyme on SH-SY5Y Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2721-2728.
[9]	WANG Jianlin, JIANG Zhiyao, LUAN Qingdong, YIN Yanbo. Comparative Pathological Analysis of SPF Chicken Host Infected with Serotype 4, 8a, 8b and 11 Fowl Adenovirus Popular in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1598-1607.
[10]	WANG Jingxuan, XU Yao, WANG Haoran, LIU Zhiyuan, ZHAO Qida, LIU Ruifang, WANG Xinyu, LIU Tao, SONG Xudong, XIAO Jianhua. A New Classification of Canine Endometritis: An Anatomical and Histopathological Perspective [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1259-1269.
[11]	XIANG Yi, ZHANG Hua, WANG Li, WEI Yong, EMU Quzhe. Effects of Aspergillus terreus on Oxidative Damage and Ferroptosis Related Indicators in Mice Liver [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3619-3626.
[12]	TAN Tianyu, CAI Dongjie, GOU Liping, REN Zhihua, ZUO Zhicai. Molecular Mechanism of High Glucose Affecting Proinflammatory Cytokine Release from Bovine Alveolar Macrophages Based on RAGE-TLR4 Crosstalk [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2944-2952.
[13]	ZHANG Jinhua, GAO Mingxing, LIU Zelin, GU Changqin. Effects of Autophagy Regulators on Apoptosis in the Brain of Mice Infected with Japanese Encephalitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 506-514.
[14]	LUO Chuanzhen, CHEN Mengyue, LOU Jiangcheng, ZHAO Hongli, ZHOU Yi, GUO Panpan, HU Xueying. Pathological Diagnosis of Two Cases of Canine Cholangiocellular Carcinoma [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 644-648.
[15]	WANG Junli, LI Yubao, DUAN Shihao, LU Jianbiao, DING Fei, SI Zhenshu, LI Xuyong, JIA Kejin, ZHANG Yunfei. Study on the Distribution of HSP60 in the MD Tumor Tissues and the Biological Function [J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2529-2536.

Cold-inducible RNA Binding Protein Involves in Response to LPS in Mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments